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However, in distinction to anticipations, NCAM1 polysialylation was not prevented in PrP ko cells but arrived at even higher levels--the latter presumably a consequence of the greater levels of NCAM1 substrate expressed in these clones. To investigate the scope of PrP's impact on NCAM1 polysialylation, we following in contrast PSA-NCAM1 levels in selected mind regions and sciatic nerves in litterm